ON RESEARCH (ANKOSH R. WANOLE) HPTLC (High-Performance Thin Layer Chromatography) INTRODUCTION High-performance Thin Layer Chromatography (HPTLC) is a sophisticated and automated form of thin-layer chromatography (TLC) with better and more advanced separation efficiency and detection limits. HPTLC is a well-known and versatile separation method, which shows many advantages compared to other separation techniques. The basic difference between conventional TLC and HPTLC is only the sorbents’ particle and pore sizes. PRINCIPLE HPTLC has a similar approach and employs the same physical principles of TLC (adsorption chromatography) i.e. the principle of separation is adsorption. The mobile phase solvent flows through because of capillary action. The components move according to their affinities towards the adsorbent. The component with more affinity towards the stationary phase travels slower. The components with the lesser affinity towards the stationary...